Research Focus
I investigate white adipose tissue plasticity and cellular crosstalk in the development and regression of obesity.
Key Findings
- 馃К Single-nucleus RNA-seq recovers all major adipose cell types and enables profiling of cellular heterogeneity from fresh and frozen WAT.
- 馃搳 Cellular and molecular memory of the obese state, particularly inflammation, persists after weight loss.
- 馃敩 Established and implemented 3D histology to study adipose tissue remodeling in obesity and weight loss.
Future Plans
I'm planning to complete my ongoing study on adipose tissue regression in the group and then transition into a role in R&D within the industry, where I aim to apply my expertise in functional genomics and tissue plasticity to drive innovation and contribute to cutting-edge developments.
Quote
馃専 "Science is nothing without the people"
Detailed Research Summary
My PhD research focused on the cellular and molecular dynamics of white adipose tissue (WAT) during obesity development and regression, using a mouse model of diet-induced obesity. I employed an integrated approach, including metabolic profiling, histology, bulk RNA-seq, single-nucleus RNA-seq, and the implementation and optimisation of 3D histology to explore WAT plasticity. My work revealed novel adipocyte subtypes, persistent inflammation after weight loss, and developed a robust protocol for single-cell profiling of adipose tissue. These findings provide critical insights into the memory of the obese state and the complex tissue remodelling during obesity and weight loss.
Key publications:
Plasticity of Epididymal Adipose Tissue in Response to Diet-Induced Obesity at Single-Nucleus Resolution
Anitta Kinga Sárvári#, Elvira Laila Van Hauwaert#, Lasse K. Markussen, Ellen Gammelmark, Ann-Britt Marcher, Morten Frendø Ebbesen, Ronni Nielsen, Jonathan Richard Brewer, Jesper Grud Skat Madsen, Susanne Mandrup. Cell Metabolism (2021), # These authors contributed equally
Isolation of Nuclei from Mouse White Adipose Tissues for Single-Nucleus Genomics
Elvira Laila Van Hauwaert, Ellen Gammelmark, Anitta Kinga Sárvári, Lena Larsen, Ronni Nielsen, Jesper Grud Skat Madsen, Susanne Mandrup. STAR Protocols (2021)
Plasticity of White Adipose Tissue Depots at Single-Cell Level during Obesity Regression in Mice
Ellen Gammelmark Klinggaard, Babukrishna Maniyadath, Flora Reincke Fuglsang, Lukas Mc.Kenzie Oussoren, Raquel Saborano, Felix Boel, Vyacheslav Akimov, Kenneth Lewis, Daniel Hansen, Kim Ravnskjær, Ormond MacDougald, Blagoy Blagoev, Søren Fisker Schmidt, Susanne Mandrup. Manuscript in preparation.
Grants:
Employed by ATLAS with PhD scholarship from the Danish Diabetes Academy 2020
Contact Information
- Email: ellen@bmb.sdu.dk
- LinkedIn: